4YYH
Crystal structure of BRD9 Bromodomain bound to a crotonyllysine peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.147, 35.700, 65.019 |
| Unit cell angles | 90.00, 99.01, 90.00 |
Refinement procedure
| Resolution | 29.920 - 1.740 |
| R-factor | 0.2142 |
| Rwork | 0.212 |
| R-free | 0.25980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hme |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.001 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.800 |
| High resolution limit [Å] | 1.740 | 3.750 | 1.740 |
| Rmerge | 0.050 | 0.035 | 0.573 |
| Total number of observations | 108728 | ||
| Number of reflections | 29841 | ||
| <I/σ(I)> | 11.8 | ||
| Completeness [%] | 99.8 | 98.7 | 100 |
| Redundancy | 3.6 | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 0.1 M sodium citrate, pH 5.5, 0.2 M sodium chloride, 20% w/v PEG3350 |
