4YY3

30S ribosomal subunit- HigB complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 41 21 2
Unit cell lengths	402.594, 402.594, 176.035
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.158 - 3.600
R-factor	0.2156
Rwork	0.214
R-free	0.23870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j5e
RMSD bond length	0.007
RMSD bond angle	1.228
Data scaling software	XDS
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.730
High resolution limit [Å]	3.600	3.600
Rmerge	0.098	0.614
Number of reflections	163739
<I/σ(I)>	9.8	1.8
Completeness [%]	98.6	99.6
Redundancy	3	3
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	14% MPD, 0.2 M KCL , 75 mM NH4Cl, 15 mM MgCl2 , 0.1M MES