4YXB
FliM(SPOA)::FliN fusion protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-09-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.150, 81.500, 89.960 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.078 - 2.560 |
R-factor | 0.2218 |
Rwork | 0.217 |
R-free | 0.25930 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.307 |
Data scaling software | Aimless (0.2.7) |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 57.670 | 57.670 | 2.670 |
High resolution limit [Å] | 2.560 | 8.870 | 2.560 |
Rmerge | 0.097 | 0.058 | 1.215 |
Rpim | 0.027 | 0.017 | 0.332 |
Total number of observations | 254307 | 6186 | 31740 |
Number of reflections | 18420 | ||
<I/σ(I)> | 18.5 | 47.2 | 2.7 |
Completeness [%] | 100.0 | 98.7 | 100 |
Redundancy | 13.8 | 12.1 | 14.3 |
CC(1/2) | 0.999 | 0.998 | 0.814 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | FliM(245-334)::FliN(5-137) was concentrated to 7.5mg/mL and crystallized with 2.2M NaCl, 100mM imidazole.Cl pH=8.0. Crystals were cryoprotected with 2M NaCl, 100mM imidazole.Cl pH=8.0, 30% glycerol. |