4YWS
Thermostable enolase from Chloroflexus aurantiacus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-15 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97 |
| Spacegroup name | I 4 |
| Unit cell lengths | 146.319, 146.319, 102.989 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.760 - 2.450 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iyx |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 103.500 | 2.580 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.046 | 0.433 |
| Number of reflections | 39821 | |
| <I/σ(I)> | 27.9 | 4.4 |
| Completeness [%] | 99.7 | 98.2 |
| Redundancy | 5.6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | 0.1 M Bis-Tris propane buffer (pH 9.0), 0.21 M NaCl and 28% PEG 1500 |
