4YWK
Pyrococcus furiosus MCM N-terminal domain with Zinc-binding subdomain B deleted
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-10-12 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.958, 50.964, 52.880 |
| Unit cell angles | 96.54, 94.77, 95.45 |
Refinement procedure
| Resolution | 50.000 - 1.550 |
| R-factor | 0.21882 |
| Rwork | 0.217 |
| R-free | 0.24994 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pof |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.450 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.082 | |
| Number of reflections | 60090 | |
| <I/σ(I)> | 32 | 13 |
| Completeness [%] | 96.2 | 94.5 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 290 | 50 mM ammonium fluoride, 21% PEG 3350 |
