4YWI
F96S/L167V Double mutant of Plasmodium Falciparum Triosephosphate Isomerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-06-30 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 50.933, 53.809, 174.495 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.890 - 1.850 |
| R-factor | 0.16383 |
| Rwork | 0.162 |
| R-free | 0.20294 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m7p |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.248 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.930 | 1.940 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.076 | 0.180 |
| Number of reflections | 35109 | |
| <I/σ(I)> | 38 | 12.8 |
| Completeness [%] | 83.6 | 61.4 |
| Redundancy | 2.3 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG 1450 22%, HEPES, Li2SO4 10mM, DTT 0.5mM, NaN3 0.5mM, EDTA 0.5mM |
