4YWG
Crystal structure of 830A in complex with V1V2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-06-16 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.033180 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.725, 159.168, 170.358 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.029 - 2.998 |
| R-factor | 0.2228 |
| Rwork | 0.219 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kdm |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.417 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.029 | 3.180 |
| High resolution limit [Å] | 2.998 | 3.000 |
| Rmerge | 0.051 | 0.729 |
| Number of reflections | 35990 | |
| <I/σ(I)> | 19.16 | 2.26 |
| Completeness [%] | 99.5 | 99.6 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 296 | 16.5% polyethylene glycol 8000 and 0.1 M Tris pH 8.5 |
