4YVB

Structure of D128N streptavidin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-01-19
Detector	PSI PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	50.597, 98.950, 52.638
Unit cell angles	90.00, 112.58, 90.00

Refinement procedure

Resolution	33.970 - 1.351
R-factor	0.1289
Rwork	0.127
R-free	0.17020
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1mep
RMSD bond length	0.018
RMSD bond angle	1.770
Data reduction software	XDS
Data scaling software	SCALA (3.3.16)
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.672	34.672	1.420
High resolution limit [Å]	1.351	4.270	1.350
Rmerge		0.016	1.096
Rmeas	0.048
Rpim	0.022	0.008	0.564
Total number of observations	459497	15031	64197
Number of reflections	101243
<I/σ(I)>	16.6	60.7	1.3
Completeness [%]	97.0	98.3	93.9
Redundancy	4.5	4.5	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.05 M HEPES, pH 7.5 with 7.5% PEG 8000