4YVA
Cathepsin K co-crystallized with actinomycetes extract
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-02-13 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 56.720, 56.720, 129.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.980 - 1.800 |
| R-factor | 0.184 |
| Rwork | 0.183 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c9e |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.801 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.981 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.071 | 0.495 |
| Number of reflections | 20402 | |
| <I/σ(I)> | 18.2 | 4.2 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 9.8 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294.15 | 24% PEG 8000, 0.2M Ammonium sulfate |
