4YV3

Trimeric crystal structure of vimentin coil1B fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97895
Spacegroup name	P 1 21 1
Unit cell lengths	39.060, 60.950, 48.900
Unit cell angles	90.00, 104.33, 90.00

Refinement procedure

Resolution	47.379 - 2.000
R-factor	0.2568
Rwork	0.255
R-free	0.29620
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.487
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXDE
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		2.100
High resolution limit [Å]	2.000	9.000	2.000
Rmerge	0.091	0.054	0.895
Rmeas	0.098	0.059	0.966
Total number of observations	219408
Number of reflections	29510	299	4024
<I/σ(I)>	11.98	27.4	2.25
Completeness [%]	99.7	91.7	99.2
Redundancy	7.4		7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	10mg/ml protein in 10mM Tris-HCl pH 8, 38 mM NaCl mixed in v/v ratio 1:1 with ammonium sulphate 2.2M, sodium thiocyanate 0.2 M