4YU8
Crystal structure of Neuroblastoma suppressor of tumorigenicity 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.27800 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 37.069, 37.069, 127.856 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.620 - 1.800 |
| R-factor | 0.21605 |
| Rwork | 0.214 |
| R-free | 0.25778 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4x1j |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.306 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.073 | |
| Number of reflections | 10031 | |
| <I/σ(I)> | 59 | 2.3 |
| Completeness [%] | 99.4 | 94.5 |
| Redundancy | 18.2 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG3350 25 %w/v, Magnesium Chloride 0.2M, bis-Tris 0.1M |
