4YU3
The crystal structure of mongoose (Helogale parvula) hemoglobin at pH 8.2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-04-23 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5417 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.038, 88.947, 55.600 |
| Unit cell angles | 90.00, 115.39, 90.00 |
Refinement procedure
| Resolution | 29.820 - 2.450 |
| R-factor | 0.26708 |
| Rwork | 0.264 |
| R-free | 0.29820 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.843 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 16749 |
| <I/σ(I)> | 13.2 |
| Completeness [%] | 99.0 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 293 | 25% PEG 3350, 50mM Tris-HCl |
