4YSD
Room temperature structure of copper nitrite reductase from Geobacillus thermodenitrificans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 300 |
| Detector technology | CCD |
| Collection date | 2013-05-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.90000 |
| Spacegroup name | H 3 |
| Unit cell lengths | 116.197, 116.197, 85.553 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.530 - 1.350 |
| R-factor | 0.09795 |
| Rwork | 0.097 |
| R-free | 0.12067 |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.350 |
| Number of reflections | 94321 |
| <I/σ(I)> | 24.4 |
| Completeness [%] | 99.8 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 5.5% PEG 4000, 0.1M sodium acetate, 0.075M copper sulphate, pH 4.5 |
