4YSC
Completely oxidized structure of copper nitrite reductase from Alcaligenes faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL3 |
| Synchrotron site | SACLA |
| Beamline | BL3 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2014-11-24 |
| Detector | MPCCD |
| Wavelength(s) | 1.771 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.100, 103.800, 147.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.900 - 2.030 |
| R-factor | 0.16583 |
| Rwork | 0.164 |
| R-free | 0.20302 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.898 |
| Data reduction software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 73.900 |
| High resolution limit [Å] | 2.030 |
| Number of reflections | 61011 |
| <I/σ(I)> | 6.97 |
| Completeness [%] | 100.0 |
| Redundancy | 147 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 293 | 100 mM sodium acetate pH 4.0, 10% PEG 4000 |
