4YS3
Nucleosome disassembly by RSC and SWI/SNF is enhanced by H3 acetylation near the nucleosome dyad axis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-02 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.510, 109.620, 181.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.450 - 3.000 |
| Rwork | 0.226 |
| R-free | 0.27920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p3l |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.083 |
| Data reduction software | DENZO |
| Data scaling software | d*TREK (9.7L) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.540 | 47.540 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
| Rmerge | 0.115 | 0.055 | 0.539 |
| Total number of observations | 291877 | 27822 | 30325 |
| Number of reflections | 42733 | ||
| <I/σ(I)> | 8.8 | 21.8 | 2.5 |
| Completeness [%] | 99.7 | 97.4 | 100 |
| Redundancy | 6.78 | 6.22 | 7.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 292.15 | postassium chloride, manganese chloride, cacodylate |
