4YPL

Crystal structure of a hexameric LonA protease bound to three ADPs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	200
Detector technology	CCD
Collection date	2014-11-22
Detector	RAYONIX MX300HE
Wavelength(s)	0.9
Spacegroup name	P 21 21 2
Unit cell lengths	169.044, 169.128, 135.361
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.450
R-factor	0.23859
Rwork	0.237
R-free	0.26480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ypn 1QZM and 1RR9
RMSD bond length	0.008
RMSD bond angle	1.517
Data scaling software	HKL-2000
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.470
High resolution limit [Å]	3.450	3.450
Number of reflections	45968
<I/σ(I)>	0.168	2.53
Completeness [%]	99.5	99.6
Redundancy	5.6	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.7	295	0.1 M Tris-HCl pH 8.7, 0.1 M CaCl2, 10-13% PEG 3350