4YOZ

p107 pocket domain in complex with HPV E7 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	80
Detector technology	CCD
Collection date	2011-11-04
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.03320
Spacegroup name	C 1 2 1
Unit cell lengths	99.690, 76.550, 74.680
Unit cell angles	90.00, 120.32, 90.00

Refinement procedure

Resolution	57.195 - 2.245
R-factor	0.1888
Rwork	0.184
R-free	0.24000
RMSD bond length	0.005
RMSD bond angle	0.873
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.200	2.370
High resolution limit [Å]	2.200	2.250
Rmerge	0.073	0.314
Number of reflections	21552
<I/σ(I)>	8.6	2
Completeness [%]	93.2	94.4
Redundancy	2.2	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	Hepes, Ammonium Sulfate, PEG 400