4YOX

Crystal structure of a trimeric exonuclease PhoExo I from Pyrococcus horikoshii OT3 in complex with poly-dT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	95
Detector technology	CCD
Collection date	2014-01-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	94.737, 94.755, 105.154
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.761 - 2.050
R-factor	0.1803
Rwork	0.179
R-free	0.20750
RMSD bond length	0.008
RMSD bond angle	1.167
Data reduction software	XDS
Data scaling software	SCALA (3.3.20)
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	70.392	19.761	2.160
High resolution limit [Å]	2.050	6.480	2.050
Rmerge		0.025	0.867
Rmeas	0.084
Rpim	0.031	0.010	0.342
Total number of observations	435374	12565	64361
Number of reflections	60017
<I/σ(I)>	16.2	54.7	2.5
Completeness [%]	99.9	96.6	100
Redundancy	7.3	6.3	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.7	293	MES, PEG6000, LiCl