4YOU

Crystal structure of a trimeric exonuclease PhoExo I from Pyrococcus horikoshii OT3 at 2.20A resolution.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	95
Detector technology	CCD
Collection date	2008-10-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	81.940, 88.980, 93.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.963 - 2.200
R-factor	0.1953
Rwork	0.193
R-free	0.23290
RMSD bond length	0.003
RMSD bond angle	0.891
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000		2.260
High resolution limit [Å]	2.200	9.840	2.200
Rmerge	0.067	0.024	0.546
Rmeas	0.074	0.026	0.639
Total number of observations	196045
Number of reflections	35388	393	2530
<I/σ(I)>	18.28	60.82	2.01
Completeness [%]	99.5	85.2	97.6
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.7	293	CaCl2, Bis-Tris, Polyethylene glycol monomethyl ether 550