4YOU
Crystal structure of a trimeric exonuclease PhoExo I from Pyrococcus horikoshii OT3 at 2.20A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2008-10-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.940, 88.980, 93.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.963 - 2.200 |
| R-factor | 0.1953 |
| Rwork | 0.193 |
| R-free | 0.23290 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.891 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.260 | |
| High resolution limit [Å] | 2.200 | 9.840 | 2.200 |
| Rmerge | 0.067 | 0.024 | 0.546 |
| Rmeas | 0.074 | 0.026 | 0.639 |
| Total number of observations | 196045 | ||
| Number of reflections | 35388 | 393 | 2530 |
| <I/σ(I)> | 18.28 | 60.82 | 2.01 |
| Completeness [%] | 99.5 | 85.2 | 97.6 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 293 | CaCl2, Bis-Tris, Polyethylene glycol monomethyl ether 550 |
