4YOT

Crystal structure of a trimeric exonuclease PhoExo I from Pyrococcus horikoshii OT3 at 2.15A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	95
Detector technology	CCD
Collection date	2008-10-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	94.610, 94.820, 99.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.573 - 2.150
R-factor	0.1843
Rwork	0.182
R-free	0.22360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.949
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000		2.210
High resolution limit [Å]	2.150	9.620	2.150
Rmerge	0.084	0.043	0.634
Rmeas	0.091	0.047	0.720
Total number of observations	337105
Number of reflections	49267	548	3559
<I/σ(I)>	13.05	26.98	2.6
Completeness [%]	99.7	86.7	98.6
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.7	293	polyethylene glycol monomethyl ether 550