4YOR
Crystal structure of a trimeric exonuclease PhoExo I from Pyrococcus horikoshii OT3 at 1.52A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2010-05-17 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 112.065, 112.065, 202.282 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.696 - 1.520 |
| R-factor | 0.1607 |
| Rwork | 0.160 |
| R-free | 0.18040 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | SHARP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 87.501 | 19.696 | 1.600 |
| High resolution limit [Å] | 1.520 | 4.810 | 1.520 |
| Rmerge | 0.018 | 0.536 | |
| Rmeas | 0.035 | ||
| Rpim | 0.013 | 0.007 | 0.332 |
| Total number of observations | 490933 | 18205 | 30293 |
| Number of reflections | 74001 | ||
| <I/σ(I)> | 30.2 | 87.9 | 2.1 |
| Completeness [%] | 98.6 | 96.9 | 91.1 |
| Redundancy | 6.6 | 7.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.9 | 293 | PEG 6000 |
