4YO3
Enteroaggregative Escherichia Coli TssA N-terminal fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-28 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 134.130, 178.050, 106.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.390 - 3.370 |
| R-factor | 0.2507 |
| Rwork | 0.250 |
| R-free | 0.26140 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.180 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXDE |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.400 | 3.470 |
| High resolution limit [Å] | 3.370 | 3.370 |
| Rmerge | 0.150 | 0.720 |
| Number of reflections | 36779 | |
| <I/σ(I)> | 11.4 | 2.4 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 7.8 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 300 nL of protein at 4.7 mg/mL with 100 nL of 29% PEG 3350, 0.1 M Hepes pH 7.5 |
