4YO2
Structure of E2F8, an atypical member of E2F family of transcription factors
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-21 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97628 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 98.830, 98.830, 121.690 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.918 - 3.073 |
| R-factor | 0.272 |
| Rwork | 0.272 |
| R-free | 0.29680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cf7 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.661 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.591 | 3.182 |
| High resolution limit [Å] | 3.073 | 3.070 |
| Rmerge | 0.169 | |
| Number of reflections | 12768 | |
| <I/σ(I)> | 8.67 | |
| Completeness [%] | 97.0 | 89.2 |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.09 | 293 | Hepes, PEG 4000, ammonium sulfate, PEG 400 |
