Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2011-08-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0070 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.923, 69.405, 92.506 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.839 - 1.700 |
| R-factor | 0.1979 |
| Rwork | 0.196 |
| R-free | 0.22450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3coi |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.098 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1839)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.065 | 0.504 |
| Number of reflections | 43897 | |
| <I/σ(I)> | 29.6 | 4.1 |
| Completeness [%] | 99.3 | 99.7 |
| Redundancy | 6.7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 50 MM AMMONIUM TARTRATE, 18% PEG 3350 |
