4YM6

Crystal structure of the human nucleosome containing 6-4PP (outside)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-30
Detector	RAYONIX MX225HE
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	105.713, 109.483, 178.214
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.831 - 3.514
R-factor	0.2464
Rwork	0.244
R-free	0.28680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3afa
RMSD bond length	0.019
RMSD bond angle	1.336
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.630
High resolution limit [Å]	3.500	7.530	3.500
Rmerge	0.096	0.043	0.551
Total number of observations	174199
Number of reflections	26130
<I/σ(I)>	9.3		4.205
Completeness [%]	99.6	96.5	100
Redundancy	6.7	5.6	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	potassium cacodylate, potassium chloride, manganese chloride