4YLB
Crystal Structure of A102D mutant of hsp14.1 from Sulfolobus solfatataricus P2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-19 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97913 |
Spacegroup name | H 3 2 |
Unit cell lengths | 132.793, 132.793, 213.058 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.589 - 2.501 |
R-factor | 0.1985 |
Rwork | 0.196 |
R-free | 0.24110 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.179 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.490 |
Number of reflections | 24402 |
<I/σ(I)> | 21.5 |
Completeness [%] | 96.6 |
Redundancy | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 293 | magnesium sulfate heptahydrate, polyethylene glycol 3350 |