4YKK
Mnemiopsis leidyi ML032222a iGluR LBD D-serine complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-02 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.951, 123.753, 54.505 |
| Unit cell angles | 90.00, 111.78, 90.00 |
Refinement procedure
| Resolution | 35.128 - 1.380 |
| R-factor | 0.1449 |
| Rwork | 0.144 |
| R-free | 0.16720 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4yki |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.233 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.400 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmerge | 0.065 | 0.610 |
| Number of reflections | 115480 | |
| <I/σ(I)> | 19.5 | 1.7 |
| Completeness [%] | 99.6 | 95.6 |
| Redundancy | 3.7 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | Reservoir: 25% PEG 8K, 100 mM Cacodylate, 150 mM MgS04. Protein buffer: 100 mM NaCl, 10 mM HEPES, pH 7.0, 0.5 mM EDTA, 100 mM D-Serine, no added glycine |
