4YKD
Crystal structure of truncated cerebral cavernous malformation 2 C-terminal adaptor domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97908 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 51.360, 51.360, 137.010 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.864 - 1.932 |
| R-factor | 0.2021 |
| Rwork | 0.200 |
| R-free | 0.23460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ykc |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.406 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.310 | 2.040 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.079 | |
| Number of reflections | 8320 | |
| <I/σ(I)> | 55.5 | 30.5 |
| Completeness [%] | 97.3 | 96.7 |
| Redundancy | 21.1 | 21.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M Bis-Tris, 30%(w/v) polyethylene glycol 550, 50 mM calcium chloride |
