4YK8
Crystal structure of the Atg101-Atg13 complex from fission yeast
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2013-11-21 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0000, 1.0070 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.822, 83.389, 91.113 |
| Unit cell angles | 90.00, 106.22, 90.00 |
Refinement procedure
| Resolution | 43.740 - 3.000 |
| R-factor | 0.267 |
| Rwork | 0.267 |
| R-free | 0.28800 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.750 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 11052 | |
| <I/σ(I)> | 15.7 | 2.5 |
| Completeness [%] | 99.2 | 99.6 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 4M sodium chloride, 0.1M Tris-HCl pH 8.0, 0.2% D-fructose 1,6-diphosphate trisodium salt octahydrate, 0.2% glycerol phosphate disodium salt hydrate, 0.2% L-O-phosphoserine, 0.2% phytic acid sodium salt hydrate, 0.02 M HEPES sodium pH 6.8 |
