4YK7
Crystal structure of S-hydroxynitrile lyase from Manihot esculenta (His103Leu)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.469, 88.098, 135.213 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.600 - 2.600 |
| R-factor | 0.24107 |
| Rwork | 0.240 |
| R-free | 0.26226 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.010 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.600 | 2.670 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.144 | 0.382 |
| Number of reflections | 32186 | |
| <I/σ(I)> | 34.6 | 12.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.1 | 14.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.1M DL-Maleic acid |
