4YJ6

The Crystal Structure of a Bacterial Aryl Acylamidase Belonging to the Amidase signature (AS) enzymes family

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	75.760, 75.760, 214.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.700 - 1.700
R-factor	0.1553
Rwork	0.155
R-free	0.16790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2f2a
RMSD bond length	0.014
RMSD bond angle	1.454
Data reduction software	MOSFLM
Data scaling software	MOSFLM
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall
Low resolution limit [Å]	43.700
High resolution limit [Å]	1.700
Number of reflections	69651
<I/σ(I)>	21.6
Completeness [%]	100.0
Redundancy	15.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295.15	1.2 M NaH2PO4/0.8 M K2HPO4, 0.1 M CAPS pH 10.5, 0.2 M Li2SO4, 0.1 M ZnCl2