4YI0
Structure of mouse importin a1 bound to Pom121NLS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-19 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.337, 89.753, 97.868 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.072 - 1.806 |
| R-factor | 0.1827 |
| Rwork | 0.182 |
| R-free | 0.20990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q5u |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.094 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1696) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.072 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.501 | |
| Number of reflections | 60036 | |
| <I/σ(I)> | 34.7 | 3.2 |
| Completeness [%] | 97.8 | 98.3 |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1 M sodium citrate tribasic dihydrate, 0.7 M sodium citrate tribasic dihydrate, 25 mM DTT |
