4YHV

Yeast Prp3 C-terminal fragment 325-469

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-30
Detector	RAYONIX MX-225
Wavelength(s)	0.91841
Spacegroup name	P 65
Unit cell lengths	56.079, 56.079, 86.753
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.283 - 2.000
R-factor	0.164
Rwork	0.161
R-free	0.22080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yhu
RMSD bond length	0.013
RMSD bond angle	1.278
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.120
High resolution limit [Å]	2.000	2.000
Rmerge	0.068	0.439
Number of reflections	10459
<I/σ(I)>	24.96	5.94
Completeness [%]	99.3	96.2
Redundancy	11.4	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M HEPES, pH 7.5, 10 % PEG 8000