4YH1

Structure of Human Scp1 bound to cis-proline peptidomimetic CTD phospho-Ser5 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97648
Spacegroup name	C 1 2 1
Unit cell lengths	125.115, 78.804, 62.853
Unit cell angles	90.00, 112.54, 90.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.1953
Rwork	0.193
R-free	0.24630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	2.002
Data reduction software	HKL-2000
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.200	5.970	2.200
Rmerge	0.110	0.033	0.495
Total number of observations	101636
Number of reflections	27838
<I/σ(I)>	6.3
Completeness [%]	97.0	99.6	83
Redundancy	3.7	3.8	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	30% PEG 3350, 0.2 M magnesium acetate