4YGB
Crystal structure of ERGIC-53/MCFD2, monoclinic calcium-free form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2013-11-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.924, 116.930, 58.065 |
| Unit cell angles | 90.00, 120.13, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| R-factor | 0.181 |
| Rwork | 0.180 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a4u |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.437 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.059 | 0.466 |
| Number of reflections | 87258 | |
| <I/σ(I)> | 39.2 | 3.4 |
| Completeness [%] | 98.9 | 100 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.2 M sodium malonate, 0.5% (v/v) Jeffamine ED-2001, 50 mM Tris-HCl |
