4YGA
CDPK1, from Toxoplasma gondii, bound to inhibitory VHH-1B7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 1 |
| Unit cell lengths | 76.920, 91.067, 106.641 |
| Unit cell angles | 88.71, 108.27, 90.26 |
Refinement procedure
| Resolution | 70.670 - 2.940 |
| R-factor | 0.227 |
| Rwork | 0.226 |
| R-free | 0.26190 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.775 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 73.040 |
| High resolution limit [Å] | 2.940 |
| Number of reflections | 53985 |
| <I/σ(I)> | 10.3 |
| Completeness [%] | 93.5 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 25% w/v PEG 3350, 0.2 M ammonium sulfate, 0.1 M Bis-Tris pH 6.5, 4% v/v 1-propanol |
