4YFW
Structural basis of glycan recognition in neonate-specific rotaviruses
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-08-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.977408 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.530, 71.940, 58.050 |
Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
Resolution | 36.530 - 1.660 |
R-factor | 0.2217 |
Rwork | 0.220 |
R-free | 0.25480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4drv |
RMSD bond length | 0.008 |
RMSD bond angle | 1.130 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.530 | 1.750 |
High resolution limit [Å] | 1.660 | 1.660 |
Rmerge | 0.080 | 0.278 |
Number of reflections | 34723 | |
<I/σ(I)> | 11.3 | 4.3 |
Completeness [%] | 98.1 | 97.2 |
Redundancy | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 20% PEG 8000 0.1 M CHES |