4YFT

HUab-20bp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	93.15
Detector technology	CCD
Collection date	2013-01-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 42 21 2
Unit cell lengths	83.036, 83.036, 63.114
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.720 - 2.914
R-factor	0.2162
Rwork	0.215
R-free	0.25110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mul
RMSD bond length	0.010
RMSD bond angle	1.110
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	BUSTER-TNT (BUSTER 2.10.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.036	2.924
High resolution limit [Å]	2.914	2.914
Rmerge	0.153	2.187
Total number of observations	141920
Number of reflections	5183
<I/σ(I)>	23.2	2.188
Completeness [%]	99.9	100
Redundancy	27.4	29.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	298	0.1M Bis-Tris pH 6.5, 30% PEG MME 550, 0.05 M CaCl2