Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 65 |
Unit cell lengths | 136.592, 136.592, 33.315 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.010 - 2.150 |
R-factor | 0.18978 |
Rwork | 0.188 |
R-free | 0.21747 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lsg |
RMSD bond length | 0.017 |
RMSD bond angle | 1.707 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | EPMR |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.010 | 2.190 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.110 | 0.918 |
Number of reflections | 19790 | |
<I/σ(I)> | 17 | 1.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 4.8 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 100 mM Bis-Tris, 20% w/v PEG5000 MME, 20% ethanol |