4YEQ

L4b Domain of Human Laminin alpha-2

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979588, 0.979804, 0.977735
Spacegroup name	P 63 2 2
Unit cell lengths	161.107, 161.107, 78.929
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.899 - 3.200
R-factor	0.2162
Rwork	0.212
R-free	0.27860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yep
RMSD bond length	0.012
RMSD bond angle	1.367
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1688))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.900	3.314
High resolution limit [Å]	3.200	3.200
Number of reflections	10031
<I/σ(I)>	15.9	1.8
Completeness [%]	96.6	94.8
Redundancy	9.6	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	100 mM sodium malonate, 100 mM MgCl2, 10% PEG 200, 100 mM Tris, pH 7.0
1	VAPOR DIFFUSION, HANGING DROP	7	293	100 mM sodium malonate, 100 mM MgCl2, 10% PEG 200, 100 mM Tris, pH 7.0