4YEP

L4b Domain of Human Laminin alpha-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.919
Spacegroup name	P 21 21 2
Unit cell lengths	57.710, 131.510, 44.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.846 - 1.190
R-factor	0.1291
Rwork	0.127
R-free	0.16190
RMSD bond length	0.022
RMSD bond angle	1.904
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.850	1.232
High resolution limit [Å]	1.190	1.190
Number of reflections	110147
<I/σ(I)>	12.2	2
Completeness [%]	99.9	99.7
Redundancy	13	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	15% PEG 3350, 20% ethylene glycol, 50 mM sodium phosphate pH 8.0