4YE4

Crystal Structure of Neutralizing Antibody HJ16 in Complex with HIV-1 gp120

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	200
Detector technology	CCD
Collection date	2013-04-14
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	I 2 2 2
Unit cell lengths	87.712, 99.037, 180.426
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.900 - 2.720
R-factor	0.1896
Rwork	0.187
R-free	0.24180
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.900
High resolution limit [Å]	2.720	2.720
Number of reflections	20510
<I/σ(I)>	1.98
Completeness [%]	94.4
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M imidazole pH 6.5 10 % (W/V) PEG 8000 5% isopropanol