4YE0
Stress-induced protein 1 truncation mutant (43 - 140) from Caenorhabditis elegans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-08 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.390, 50.820, 130.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.950 - 2.100 |
| R-factor | 0.23171 |
| Rwork | 0.230 |
| R-free | 0.25948 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.060 | 0.527 |
| Number of reflections | 14754 | |
| <I/σ(I)> | 16.3 | 2.9 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 293 | 33 mM CAPS, 17 mM BisTRIS, 17 mM ammoniumsulfate, 10% pentaerythritol ethoxylate |
