4YDT
Beta1 synthetic solenoid protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.04030 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 102.370, 102.370, 290.430 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 75.670 - 3.310 |
| R-factor | 0.2571 |
| Rwork | 0.255 |
| R-free | 0.30299 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.564 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.670 | 3.400 |
| High resolution limit [Å] | 3.310 | 3.310 |
| Rmerge | 0.160 | 0.950 |
| Number of reflections | 8605 | |
| <I/σ(I)> | 5.2363 | 1.08 |
| Completeness [%] | 94.8 | 96.87 |
| Redundancy | 7.71 | 7.92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 160 mM magnesium chloride, 80 mM sodium cacodylate pH 6.5, 40% v/v PEG 200 |
