4YCX

Binary complex of human DNA Polymerase Mu with 2-nt gapped DNA substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	93
Detector technology	CCD
Collection date	2012-09-01
Detector	RIGAKU SATURN 92
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	60.043, 68.683, 116.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.470 - 2.100
R-factor	0.203
Rwork	0.201
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lzd
RMSD bond length	0.002
RMSD bond angle	0.565
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	28624
<I/σ(I)>	9.8	2.94
Completeness [%]	99.9	100
Redundancy	6.6	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	294	0.1M Tris, 20% PEG 8K, 0.2M MgCl2