4Y90
Crystal structure of Triosephosphate Isomerase from Deinococcus radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-03-04 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 169.620, 169.620, 202.266 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.133 - 2.100 |
| R-factor | 0.1382 |
| Rwork | 0.136 |
| R-free | 0.18560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yya |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.296 |
| Data reduction software | CrystalClear |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.133 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.097 | 0.464 |
| Number of reflections | 64962 | |
| <I/σ(I)> | 11 | 2.9 |
| Completeness [%] | 99.7 | 98.6 |
| Redundancy | 4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291.15 | 1.8 M ammonium sulfate, 0.1 M sodium citrate tribasic pH 5.6, 0.2 M potassium sodium tartrate |
