4Y8F

Crystal structure of Triosephosphate Isomerase from Clostridium perfringens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-02-29
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	75.255, 49.555, 71.502
Unit cell angles	90.00, 119.11, 90.00

Refinement procedure

Resolution	37.589 - 1.540
R-factor	0.1484
Rwork	0.147
R-free	0.18130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1btm
RMSD bond length	0.010
RMSD bond angle	1.239
Data reduction software	CrystalClear
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1313))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.589	1.620
High resolution limit [Å]	1.540	1.540
Rmerge	0.062	0.448
Number of reflections	33930
<I/σ(I)>	14.6	3.1
Completeness [%]	99.2	94.4
Redundancy	4.2	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	291.15	0.2 M ammonium acetate, 0.1 M sodium acetate pH 4.6, 30% w/v polyethylene glycol (PEG) 4000