4Y88
Crystal structure of the N-terminal domain of CEACAM8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.03317 |
Spacegroup name | P 41 3 2 |
Unit cell lengths | 94.336, 94.336, 94.336 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 66.710 - 1.450 |
R-factor | 0.1467 |
Rwork | 0.145 |
R-free | 0.17980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qsq |
RMSD bond length | 0.011 |
RMSD bond angle | 1.505 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.710 | 1.470 |
High resolution limit [Å] | 1.450 | 1.450 |
Number of reflections | 26074 | |
<I/σ(I)> | 27 | 1.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 48.9 | 49 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 293 | 1.2M Ammmonium Sulphate, 0.1M Citric Acid, pH 3.5 |