4Y7K
Structure of an archaeal mechanosensitive channel in closed state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 87.292, 140.368, 182.544 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.000 - 3.500 |
| R-factor | 0.26085 |
| Rwork | 0.259 |
| R-free | 0.28709 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oar 2b98 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.568 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.630 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 1.000 | |
| Number of reflections | 27942 | |
| <I/σ(I)> | 26.2 | 1.8 |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 11 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.3 | 289 | 7% PEG4000, 0.4M NH4SCN, 0.1M Citric acid(pH7.3) |
