4Y7J
Structure of an archaeal mechanosensitive channel in expanded state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 147.360, 149.250, 99.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 4.100 |
| R-factor | 0.32335 |
| Rwork | 0.321 |
| R-free | 0.37582 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b98 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.116 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 4.200 |
| High resolution limit [Å] | 4.100 | 4.100 |
| Rmerge | 0.109 | 0.938 |
| Number of reflections | 17722 | |
| <I/σ(I)> | 8.7 | 2.3 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 9 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 1.6M (NH4)2SO4, 0.1M NaCl, 0.1M HEPES |
